研究業績(Publications)

原著論文(Original articles)

2021年-

  • [preprint] Sakai Y#, Ariyoshi M#, Hirano Y# (#共同第一著者), Suzuki A, Hirota T, Tachikawa M, Fukagawa T. “CCAN forms the spherical shell-like structure in interphase kinetochores” bioRxiv, DOI: 10.64898/2026.01.14.699427 (2026)
  • Kong W, Hara M, Tokunaga Y, Okumura K, Hirano Y, Miao J, Takenoshita Y, Hashimoto M, Sasaki H, Fujimori T, Wakabayashi Y, Fukagawa T. “CENP-C-Mis12 complex establishes a regulatory loop through Aurora B for chromosome segregation” Life Sci. Alliance, 8(1): e202402927 (2024) DOI: 10.26508/lsa.202402927
  • Hirano Y(*責任著者), Sato T, Miura A, Kubota Y, Shindo K, Fukase K, Fukagawa T, Kabayama K, Haraguchi T, Hiraoka Y. “Disordered region of nuclear membrane protein Bqt4 recruits phosphatidic acid to the nuclear envelope to maintain its structural integrity” J. Biol. Chem, 300(7): 107430 (2024) DOI: 10.1016/j.jbc.2024.107430
  • Nagpal H, Ali-Ahmad A, Hirano Y, Cai W, Halic M, Fukagawa T, Sekulic N, Firez B. “CENP-A and CENP-B collaborate to create an open centromeric chromatin state” Nat. Commun., 14(1): 8227 (2023) DOI: 10.1038/s41467-023-43739-5
  • Le TK, Hirano Y, Asakawa H, Okamoto K, Fukagawa T, Haraguchi T, Hiraoka Y. “A ubiquitin–proteasome pathway degrades the inner nuclear membrane protein Bqt4 to maintain nuclear membrane homeostasis” J. Cell Sci., 136(19): jcs260930 (2023) DOI: 10.1242/jcs.260930
  • Hirano Y(*責任著者), Kinugasa Y, Kubota Y, Obuse C, Haraguchi T, Hiraoka Y*. “Inner nuclear membrane proteins Lem2 and Bqt4 interact with different lipid synthesis enzymes in fission yeast” J. Biochem., 174(1): 33–46 (2023) DOI: 10.1093/jb/mvad017
    Journal cover, 第32回JB論文賞に選出
  • Hirano Y(*責任著者), Ohno Y, Kubota Y, Fukagawa T, Kihara A, Haraguchi T, Hiraoka Y*. “Ceramide synthase homolog Tlc4 maintains nuclear envelope integrity via its Golgi translocation” J. Cell Sci., 136(10): jcs260923 (2023) DOI: 10.1242/jcs.260923
    First personとして紹介された
  • Watabe M, Hirano Y, Iwane A, Matoba O, Takahashi K. “Optical dispersions through intracellular inhomogeneities” Phys. Rev. Res., 5(2): L022043 (2023) DOI: 10.1103/PhysRevResearch.5.L022043
  • Asakawa H, Hirano Y, Shindo T, Haraguchi T, Hiraoka Y. “Fission yeast Ish1 and Les1 interact with each other in the lumen of the nuclear envelope” Genes Cells, 27(11): 643-656 (2022) DOI: 10.1111/gtc.12981
  • Hiraoka H, Wang J, Nakano T, Hirano Y, Yamazaki S, Hiraoka Y, Haraguchi T. “ATP levels influence cell movement during the mound phase in Dictyostelium discoideum as revealed by ATP visualization and simulation” FEBS Open Bio., 12(11): 2042-2056 (2022) DOI: 10.1002/2211-5463.13480
  • Martin Caballero L, Capella M, Barrales RR, Dobrev N, van Emden T, Hirano Y, Suma Sreechakram VN, Fischer-Burkart S, Kinugasa Y, Nevers A, Rougemaile M, Sinnig I, Fischer T, Hiraoka Y, Braun S. “The inner nuclear membrane protein Lem2 coordinates RNA degradation at the nuclear periphery” Nat. Struct. Mol. Biol., 29, 910-921 (2022) DOI: 10.1038/s41594-022-00831-6
  • Watanabe R, Hirano Y, Hara M, Hiraoka Y, Fukagawa T. “Mobility of kinetochore proteins measured by FRAP analysis in living cells” Chromosome Res., 30(1): 43-57 (2022) DOI: 10.1007/s10577-021-09678-x
  • Haraguchi T, Koujin T, Shindo T, Bilir S, Osakada H, Nishimura K, Hirano Y, Asakawa H, Mori C, Kobayashi S, Okada Y, Chikashige Y, Fukagawa T, Shibata S, Hiraoka Y. “Transfected plasmid DNA is incorporated into the nucleus via nuclear envelope reformation at telophase” Commun. Biol., 5(1):78 (2022) DOI: 10.1038/s42003-022-03021-8
  • Hayashi-Takanaka Y, Hayashi Y, Hirano Y, Miyawaki-Kuwakado A, Ohkawa Y, Obuse C, Kimura H, Haraguchi T, Hiraoka Y. “Chromatin loading of MCM hexamers is associated with di-/tri-methylation of histone H4K20 toward S phase entry” Nucleic Acids Res., 49(21): 12152-12166 (2021) DOI: 10.1093/nar/gkab1068

2003-2020年

  • Hirano Y(*責任著者), Kinugasa Y, Osakada H, Shindo T, Kubota Y, Shibata S, Haraguchi T, Hiraoka Y*. “Lem2 and Lnp1 maintain the membrane boundary between the nuclear envelope and endoplasmic reticulum” Commun. Biol., 3(1):276 (2020) DOI : 10.1038/s42003-020-0999-9
  • Hiraoka H, Nakano T, Kuwana S, Fukuzawa M, Hirano Y, Ueda M, Haraguchi T, Hiraoka Y. “Intracellular ATP levels influence cell fates in Dictyostelium discoideum differentiation” Genes Cells, 25(5): 312-32 (2020) DOI: 10.1111/gtc.12763
  • Osemwenkhae OP, Sakuno T, Hirano Y, Asakawa H, Hayashi-Takanaka Y, Haraguchi T, Hiraoka Y. “Human Ebp1 rescues the synthetic lethal growth of fission yeast cells lacking Cdb4 and Nup184” Genes Cells, 25(4): 288-295 (2020) DOI: 10.1111/gtc.12757
  • Kinugasa Y, Hirano Y, Sawai M, Ohno Y, Shindo T, Asakawa H, Chikashige Y, Shibata S, Kihara A, Haraguchi T, Hiraoka Y. “The very-long-chain fatty acid elongase Elo2 rescues lethal defects associated with loss of the nuclear barrier function” J. Cell Sci., 132(10): jcs229021 (2019) DOI: 10.1242/jcs.229021
    Research Highlightsで紹介された
  • Hirano Y, Kinugasa Y, Asakawa H, Chikashige Y, Obuse C. Haraguchi T, Hiraoka Y. “Lem2 is retained at the nuclear envelope through its interaction with Bqt4 in fission yeast” Genes Cells, 23(3): 122-135 (2018) DOI: 10.1111/gtc.12557
    Top downloaded article 2017-2018 in this journal:a top 20 most read paperに選出
  • Matsuda A, Schermelleh L, Hirano Y, Haraguchi T, Hiraoka Y. “Accurate and fiducial-marker-free correction for three-dimensional chromatic shift in biological fluorescence microscopy” Sci. Rep., 8(1):7583 (2018) DOI: 10.1038/s41598-018-25922-7
    Top 100 most accessed papers in Cell and Molecular Biologyに選出
  • Hirai Y, Hirano Y, Matsuda A, Hiraoka Y, Honda T, Tomonaga K. “Borna Disease Virus Assembles Porous Cage-like Viral Factories in the Nucleus” J. Biol. Chem., 291(50): 25789-25798 (2016) DOI: 10.1074/jbc.M116.746396
  • Tange Y, Chikashige Y, Takahata S, Kawakami K, Higashi M, Mori C, Kojidani T, Hirano Y, Asakawa H, Murakami Y, Haraguchi T, Hiraoka Y. “Inner nuclear membrane protein Lem2 augments heterochromatin formation in response to nutritional conditions” Genes Cells, 21(8), 812-832 (2016) DOI: 10.1111/gtc.12385
  • Gómez-Saldivar G, Fernandez A, Hirano Y. Mauro M, Lai A, Ayuso C, Haraguchi T, Hiraoka Y, Piano F, Askjaer P. “Identification of Conserved MEL-28/ELYS Domains with Essential Roles in Nuclear Assembly and Chromosome Segregation” PLoS Genet., 12(6): e1006131 (2016) DOI: 10.1371/journal.pgen.1006131
  • Hirano Y, Hizume K, Kimura H, Takeyasu K, Haraguchi T, Hiraoka Y. “Lamin B receptor recognizes specific modifications of histone H4 in heterochromatin formation” J. Biol. Chem., 287(51), 42654-42663 (2012) DOI: 10.1074/jbc.M112.397950
  • Ito S, Tan LJ, Andoh D, Narita T, Seki M, Hirano Y, Narita K, Kuraoka I, Hiraoka Y, Tanaka K. “MMXD, a TFIIH-independent XPD-MMS19 protein complex involved in chromosome segregation”Mol. Cell, 27(4), 632-640 (2010) DOI: 10.1016/j.molcel.2010.07.029
  • Hirano Y(*責任著者), Iwase Y, Ishii K, Kumeta M, Horigome T, Takeyasu K. “Cell cycle-dependent phosphorylation of MAN1” Biochemistry, 48(7), 1636-1643 (2009) DOI: 10.1021/bi802060v
  • Hirano Y(*責任著者), Ishii K, Kumeta M, Furukawa K, Takeyasu K Horigome T. “Proteomic and targeted analytical identification of BXDC1 and EBNA1BP2 as dynamic scaffold proteins in the nucleolus” Genes Cells, 14(2), 155-166 (2009) DOI: 10.1111/j.1365-2443.2008.01262.x
  • Ishii K, Hirano Y, Araki N, Oda T, Kumeta M, Takeyasu K, Furukawa K, Horigome T. “Nuclear matrix contains novel WD-repeat and disordered-region-rich proteins” FEBS Lett., 582(23-24), 3515-3519 (2008) DOI: 10.1016/j.febslet.2008.09.019
  • Hirano Y(*責任著者), Ohniwa RL, Wada C, Yoshimura SH, Takeyasu K. “Human small G proteins, ObgH1, and ObgH2, participate in the maintenance of mitochondria and nucleolar architectures” Genes Cells, 11(11), 1295-1304 (2006) DOI: 10.1111/j.1365-2443.2006.01017.x
  • Hirano Y, Segawa M, Ouchi FS, Yamakawa Y, Furukawa K, Takeyasu K, Horigome T. “Dissociation of emerin from barrier-to-autointegration factor is regulated through mitotic phosphorylation of emerin in Xenopus egg cell-free system” J. Biol. Chem., 280(48), 39925-39933 (2005) DOI: 10.1074/jbc.M503214200
  • Miyachi K, Hirano Y, Horigome T, Mimori K, Miyakawa H, Onozuka Y, Shibata M, Hirakata M, Suwa A, Hosaka H, Matsushima S, Komatsu T, Matsushima H, Hankins RW, Fritzler M.J. “Autoantibodies from primary biliary cirrhosis patients with anti-p95c antibodies bind to recombinant p97/VCP and inhibit in vitro nuclear envelope assembly” Clin. Exp. Immunol., 136(3), 568-573 (2004) DOI: 10.1111/j.1365-2249.2004.02456.x
  • Nakagawa T#, Hirano Y#(#共同第一著者), Inomata A, Yokota S, Miyachi K, Kaneda M, Umeda M, Furukawa K, Omata S, Horigome T. “Participation of a Fusogenic Protein, Glyceraldehyde-3-Phosphate Dehydrogenase, in Nuclear Membrane Assembly” J. Biol. Chem., 278(22), 20395-20404 (2003) DOI: 10.1074/jbc.M210824200

総説(査読有)

  • Asakawa H, Hirano Y, Haraguchi T. “Nuclear Organization (Nuclear Structure and Dynamics)” Encyclopedia of Cell Biology, 2nd Edition, 2, 408-41 (2023)
  • Hirano Y, Asakawa H, Sakuno T, Haraguchi T, Hiraoka Y. “Nuclear Envelope Proteins Modulating the Heterochromatin Formation and Functions in Fission Yeast” Cells, 9(8):E1908 (2020)
  • Hirano Y, Matsuda A, Hiraoka Y. “Recent advancements in structured-illumination microscopy toward live-cell imaging” Microscopy(Oxf), 64(4), 237-249 (2015)
  • Hirano Y, Takahashi H, Kumeta M, Hizume K, Ohtsuka S, Yoshimura SH, Takeyasu K. “Nuclear architecture and chromatin dynamics revealed by atomic force microscopy in combination with biochemistry and cell biology” Pflugers Arch., 456(1), 139-153 (2008)

著書

  • 平野泰弘,松田厚志,平岡泰「実践編3. 3D-SIM による細胞内構造の超解像イメージング」初めてでもできる!超解像イメージング、146-156、羊土社　2016年
  • 松田厚志、平野泰弘、平岡泰「原理・応用編3.　構造化照明顕微鏡法SIM―縞照明のつくるモアレが可能にする超解像観察」初めてでもできる!超解像イメージング 219-228、羊土社　2016年
  • 平野泰弘、松田厚志「超分解蛍光顕微鏡法」新・生細胞蛍光イメージング、第7章、共立出版　2015年
  • 松田厚志、原口徳子、平野泰弘「超高分解蛍光顕微鏡法=解像度の限界を超えて見る=」光アライアンス、3, 31-35、日本工業出版　2014年
  • 松田厚志、平野泰弘、平岡泰「核内構造観察のための超分解能顕微鏡イメージング技術」細胞工学、31, 863-869、学研メディカル秀潤社　2012年
  • 平野泰弘、平岡泰「ヒストンH1」生体の化学、62, 438-439、医学書院　2011年
  • 平野泰弘、高橋寛英、吉村成弘、竹安邦夫「原子間力顕微鏡を用いた力学測定と分子認識イメージング」生命科学者のための機器分析実験ハンドブック、116-121、羊土社2007年
  • Horigome T, Hirano Y and Furukawa K “Dynamics, roles and diseases of the nuclear membrane, lamins and lamin-binding proteins” Nuclear Dynamics, 123-144, Springer (2007)