{"id":447,"date":"2025-04-11T12:16:29","date_gmt":"2025-04-11T03:16:29","guid":{"rendered":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/?p=447"},"modified":"2025-04-11T12:16:29","modified_gmt":"2025-04-11T03:16:29","slug":"gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e8%ab%96%e6%96%87%e3%81%8canalytical-biochemistry%e8%aa%8c%e3%81%ab%e6%8e%b2","status":"publish","type":"post","link":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2025\/04\/11\/gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e8%ab%96%e6%96%87%e3%81%8canalytical-biochemistry%e8%aa%8c%e3%81%ab%e6%8e%b2\/","title":{"rendered":"GFP\u306e\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u8ad6\u6587\u304cAnalytical Biochemistry\u8a8c\u306b\u63b2\u8f09"},"content":{"rendered":"<p>bioRxiv\u3067\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3092\u516c\u958b\u3057\u3066\u3044\u305f\u3001GFP\u306e\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ad6\u6587\u304c<a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0003269725000995\">Analytical Biochemistry\u8a8c<\/a>\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002\u751f\u5316\u5b66\u7684\u306a\u5b9f\u9a13\u624b\u6cd5\u306b\u91cd\u304d\u3092\u7f6e\u3044\u305f\u3001\u6614\u304b\u3089\u3042\u308b\u30b8\u30e3\u30fc\u30ca\u30eb\u3067\u3059\u3002\u6d3e\u624b\u3055\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u79c1\u306e\u6301\u3063\u3066\u3044\u308b\u8ad6\u6587\u3092\u78ba\u8a8d\u3059\u308b\u3068\u7d50\u69cb\u8aad\u3093\u3067\u304a\u308a\u3001\u5b9f\u9a13\u624b\u6cd5\u306e\u958b\u767a\u3068\u3044\u3046\u5185\u5bb9\u3082\u3001\u3053\u306e\u30b8\u30e3\u30fc\u30ca\u30eb\u306b\u30d5\u30a3\u30c3\u30c8\u3057\u3066\u3044\u305f\u304b\u3068\u601d\u3044\u307e\u3059\u3002\u30aa\u30fc\u30d7\u30f3\u30a2\u30af\u30bb\u30b9\u3067\u3059\u306e\u3067\u3001\u8cfc\u8aad\u3057\u3066\u3044\u308b\u304b\u306b\u95a2\u308f\u3089\u305a\u3001\u8ab0\u3067\u3082\u8aad\u3081\u307e\u3059\u3002<\/p>\n<p><span class=\"csl-entry\"><span class=\"csl-right-inline\">Shiratori, M.; Tsuyuki, R.; Asanuma, M.; Kawabata, S.; Yoshioka, H.; Ohgane, K. In-Gel Refolding Allows Fluorescence Detection of Fully Denatured GFPs after SDS-PAGE. <i>Anal. Biochem.<\/i> <b>2025<\/b>, <i>702<\/i>, 115861. <a href=\"https:\/\/doi.org\/10.1016\/j.ab.2025.115861\">https:\/\/doi.org\/10.1016\/j.ab.2025.115861<\/a>.<\/span><\/span><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-448\" src=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-1024x597.png\" alt=\"\" width=\"640\" height=\"373\" srcset=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-1024x597.png 1024w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-300x175.png 300w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-768x447.png 768w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-1536x895.png 1536w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-2048x1193.png 2048w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-24x14.png 24w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-36x21.png 36w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2025\/04\/8c62808aa3795eb5f2ce52f3be12bcee-48x28.png 48w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p>\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3068\u3057\u3066\u67fb\u8aad\u524d\u306e\u539f\u7a3f\u306fbioRxiv\u3067\u516c\u958b\u6e08\u307f\u3067\u3059\u304c\u3001\u67fb\u8aad\u3092\u7d4c\u3066\u307b\u307c\u5168\u3066\u306e\u56f3 (Figure 1-5)\u304c\u65b0\u305f\u306a\u56f3\u306b\u7f6e\u304d\u63db\u308f\u308a\u3001\u8cea\u3082\u9ad8\u304f\u306a\u3063\u3066\u3044\u308b\u304b\u3068\u601d\u3044\u307e\u3059\u306e\u3067\u3001\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3067\u306f\u306a\u304f\u4eca\u5f8c\u306f\u67fb\u8aad\u6e08\u307f\u306e\u3053\u3061\u3089\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u3053\u306e\u3088\u3046\u306a\u56fd\u969b\u96d1\u8a8c\u3078\u306e\u8ad6\u6587\u306e\u6295\u7a3f\u3067\u306f\u3001\u307e\u305a\u30a8\u30c7\u30a3\u30bf\u30fc\u304c\u5185\u5bb9\u3092\u78ba\u8a8d\u3057\u3001\u5c02\u9580\u5bb6\u6570\u4eba\u306b\u67fb\u8aad\u3092\u4f9d\u983c\u3057\u3001\u67fb\u8aad\u8005\u304c\u8a55\u4fa1\u3092\u884c\u3044\u3001\u554f\u984c\u70b9\u3084\u4fee\u6b63\u3059\u3079\u304d\u70b9\u3092\u6307\u6458\u3057\u307e\u3059\u3002\u305d\u308c\u3089\u306e\u6307\u6458\u4e8b\u9805\u3092\u3001\u57f7\u7b46\u8005\u5074\u3067\u518d\u5b9f\u9a13\u3084\u30c7\u30fc\u30bf\u306e\u8ffd\u52a0\u3067\u5bfe\u5fdc\u3057\u3001\u5341\u5206\u306b\u8981\u6c42\u3092\u6e80\u305f\u305b\u3070\u30a2\u30af\u30bb\u30d7\u30c8\u3055\u308c\u3001\u63b2\u8f09\u3068\u3044\u3046\u6d41\u308c\u3067\u3059\u3002<\/p>\n<p>\u4eca\u56de\u3001\u6295\u7a3f\u304b\u3089\u30a2\u30af\u30bb\u30d7\u30c8\u30fb\u63b2\u8f09\u307e\u3067\u3001\u307b\u307c6\u30f6\u6708\u304c\u304b\u304b\u308a\u307e\u3057\u305f\u3002\u6700\u521d\u306e\u4e09\u30f6\u6708\u306f\u7406\u7531\u306f\u308f\u304b\u308a\u307e\u305b\u3093\u304c\u30b8\u30e3\u30fc\u30ca\u30eb\u306e\u4e8b\u52d9\u5c40\u30fb\u30a8\u30c7\u30a3\u30bf\u30fc\u306e\u3068\u3053\u308d\u3067\u539f\u7a3f\u304c\u6b62\u307e\u3063\u3066\u3044\u305f\u305f\u3081(\u3057\u3070\u3057\u3070\u8d77\u3053\u308a\u307e\u3059)\u3001\u5b9f\u8cea\u7684\u306b\u306f\u4e09\u30f6\u6708(\u3060\u3063\u305f\u306f\u305a)\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u67fb\u8aad\u8005\u4e8c\u4eba\u306e\u5bfe\u5fdc\u306f\u975e\u5e38\u306b\u65e9\u304f\u3001\u539f\u7a3f\u304c\u56de\u3063\u30661\u9031\u9593\u3067\u67fb\u8aad\u7d50\u679c\u304c\u5c4a\u304d\u307e\u3057\u305f\u3002\u67fb\u8aad\u306f\u4e8c\u4eba\u3068\u3082\u304a\u304a\u3080\u306d\u597d\u610f\u7684\u3067\u306f\u3042\u308a\u307e\u3057\u305f\u304c\u3001&#8221;\u5b9a\u91cf\u7684\u306a\u8a55\u4fa1&#8221;\u3001&#8221;\u518d\u73fe\u6027\u3092\u7d71\u8a08\u7684\u306b\u793a\u3059\u3053\u3068&#8221;\u304c\u8981\u6c42\u3055\u308c\u307e\u3057\u305f\u3002\u305d\u306e\u4ed6\u3001\u9069\u7528\u53ef\u80fd\u306aGFP\u306e\u7a2e\u985e\u306a\u3069\u306e\u70b9\u3067&#8221;too preliminary&#8221;\u3067\u306f\u306a\u3044\u304b\u3068\u3044\u3046\u30b3\u30e1\u30f3\u30c8\u3082\u3042\u308a\u307e\u3057\u305f\u304c\u3001\u3053\u308c\u307e\u3067\u306b\u77e5\u3089\u308c\u3066\u3044\u308bGFP\u306e\u5909\u7a2e\u5168\u7a2e\u985e\u3067\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ef\u80fd\u304b\u3092\u8abf\u3079\u308b\u306e\u306f\u7c21\u5358\u3067\u306f\u306a\u304f\u3001\u3069\u306eGFP\u3067\u8a66\u3059\u304b\u3082\u660e\u78ba\u306a\u57fa\u6e96\u304c\u306a\u3044\u3053\u3068\u304b\u3089\u3001\u4eca\u56de\u306e\u8ad6\u6587\u306e\u7bc4\u56f2\u5916\u3067\u3042\u308d\u3046\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002\u5b9a\u91cf\u7684\u306a\u89e3\u6790\u3068\u7d71\u8a08\u89e3\u6790\u306b\u3064\u3044\u3066\u306f\u3001\u8ad6\u6587\u306b\u8f09\u305b\u3066\u3044\u305f\u307b\u307c\u5168\u3066\u306e\u56f3\u3067(\u79c1\u305f\u3061\u306a\u308a\u306b)\u304b\u306a\u308a\u3057\u3063\u304b\u308a\u3068\u884c\u3044(2\u30f6\u6708\u534a)\u3001\u56f3\u3084\u672c\u6587\u3092\u30ea\u30d0\u30a4\u30ba(\u6539\u8a02)\u3057\u3001\u30a2\u30af\u30bb\u30d7\u30c8\u306b\u81f3\u308a\u307e\u3057\u305f\u3002<\/p>\n<p>B4\u767d\u9ce5\u3055\u3093\u306b\u306f\u5352\u7814\u767a\u8868\u76f4\u5f8c\u304b\u30893\u6708\u306b\u304b\u3051\u3066\u304b\u306a\u308a\u306e\u6570\u306e\u5b9f\u9a13 (SDS-PAGE\u30b2\u30eb\u4f5c\u6210\u3001\u96fb\u6c17\u6cf3\u52d5\u3001\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0)\u3092\u884c\u3063\u3066\u3082\u3089\u3044\u307e\u3057\u305f\u304c\u3001\u7121\u4e8b\u30a2\u30af\u30bb\u30d7\u30c8\u306b\u306a\u308a\u5831\u308f\u308c\u305f\u304b\u306a\u3001\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<p style=\"text-align: right\">\u5927\u91d1<\/p>\n<blockquote class=\"wp-embedded-content\" data-secret=\"sPA04E4ASy\"><p><a href=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2024\/10\/06\/gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e3%81%ae%e8%ab%96%e6%96%87%e3%81%8cbiorxiv%e3%81%a7%e5%85%ac%e9%96%8b\/\">GFP\u306e\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ad6\u6587\u304cbioRxiv\u3067\u516c\u958b<\/a><\/p><\/blockquote>\n<p><iframe loading=\"lazy\" class=\"wp-embedded-content\" sandbox=\"allow-scripts\" security=\"restricted\" style=\"position: absolute; visibility: hidden;\" title=\"&#8220;GFP\u306e\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ad6\u6587\u304cbioRxiv\u3067\u516c\u958b&#8221; &#8212; \u5927\u91d1\u7814\u7a76\u5ba4\" src=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2024\/10\/06\/gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e3%81%ae%e8%ab%96%e6%96%87%e3%81%8cbiorxiv%e3%81%a7%e5%85%ac%e9%96%8b\/embed\/#?secret=ixEzwM8o3A#?secret=sPA04E4ASy\" data-secret=\"sPA04E4ASy\" width=\"600\" height=\"338\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\"><\/iframe><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>bioRxiv\u3067\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3092\u516c\u958b\u3057\u3066\u3044\u305f\u3001GFP\u306e\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ad6\u6587\u304cAnalytical Biochemistry\u8a8c\u306b\u63b2\u8f09\u3055\u308c\u307e\u3057\u305f\u3002\u751f\u5316\u5b66\u7684\u306a\u5b9f\u9a13\u624b\u6cd5\u306b\u91cd\u304d\u3092\u7f6e\u3044\u305f\u3001\u6614\u304b\u3089\u3042\u308b\u30b8\u30e3\u30fc\u30ca\u30eb\u3067\u3059\u3002\u6d3e\u624b\u3055\u306f\u3042 &hellip; <a href=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2025\/04\/11\/gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e8%ab%96%e6%96%87%e3%81%8canalytical-biochemistry%e8%aa%8c%e3%81%ab%e6%8e%b2\/\">\u7d9a\u304d\u3092\u8aad\u3080 <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":48,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-447","post","type-post","status-publish","format-standard","hentry","category-ohganelabnews"],"_links":{"self":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/447","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/users\/48"}],"replies":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/comments?post=447"}],"version-history":[{"count":1,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/447\/revisions"}],"predecessor-version":[{"id":449,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/447\/revisions\/449"}],"wp:attachment":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/media?parent=447"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/categories?post=447"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/tags?post=447"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}