{"id":435,"date":"2024-10-06T10:54:23","date_gmt":"2024-10-06T01:54:23","guid":{"rendered":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/?p=435"},"modified":"2024-11-25T12:00:39","modified_gmt":"2024-11-25T03:00:39","slug":"gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e3%81%ae%e8%ab%96%e6%96%87%e3%81%8cbiorxiv%e3%81%a7%e5%85%ac%e9%96%8b","status":"publish","type":"post","link":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2024\/10\/06\/gfp%e3%81%ae%e3%82%b2%e3%83%ab%e5%86%85%e3%83%aa%e3%83%95%e3%82%a9%e3%83%bc%e3%83%ab%e3%83%87%e3%82%a3%e3%83%b3%e3%82%b0%e3%81%ae%e8%ab%96%e6%96%87%e3%81%8cbiorxiv%e3%81%a7%e5%85%ac%e9%96%8b\/","title":{"rendered":"GFP\u306e\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u306e\u8ad6\u6587\u304cbioRxiv\u3067\u516c\u958b"},"content":{"rendered":"

\u5927\u91d1\u7814\u306e\u7814\u7a76\u304c\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u30b5\u30fc\u30d0\u30fcbioRxiv\u3067\u516c\u958b\u3055\u308c\u307e\u3057\u305f\u3002B4\u767d\u9ce5\u3055\u3093\u3001M1\u9732\u6728\u3055\u3093\u3001\u5352\u696d\u751f\u306e\u5ddd\u7aef\u3055\u3093\u304c\u5bc4\u4e0e\u3057\u3066\u304f\u308c\u3066\u3044\u308b\u7814\u7a76\u3067\u3059\u3002<\/p>\n

#\u306a\u304a\u3001\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3068\u306f\u3001\u5b66\u8853\u96d1\u8a8c\u3067\u306e\u67fb\u8aad\u524d\u306e\u539f\u7a3f\u3092\u516c\u958b\u3057\u305f\u3082\u306e\u3067\u3001\u5c02\u9580\u5bb6\u306b\u3088\u308b\u67fb\u8aad\u3092\u901a\u904e\u3057\u3066\u3044\u306a\u3044\u3068\u3044\u3046\u70b9\u3067\u5b66\u8853\u96d1\u8a8c\u306b\u63b2\u8f09\u6e08\u307f\u306e\u8ad6\u6587\u3068\u306f\u7570\u306a\u308a\u307e\u3059\u3002\u67fb\u8aad\u30fb\u4fee\u6b63\u306b\u306f\u901a\u5e38\u6570\u30f6\u6708\u4ee5\u4e0a\u304b\u304b\u308b\u3053\u3068\u3001\u5b66\u8853\u96d1\u8a8c\u306f\u5951\u7d04\u3057\u3066\u3044\u306a\u3044\u3068\u8aad\u3081\u306a\u3044\u3053\u3068\u306a\u3069\u304c\u3042\u308a\u3001\u7814\u7a76\u306e\u52a0\u901f\u3068\u8ab0\u3067\u3082\u60c5\u5831\u306b\u30a2\u30af\u30bb\u30b9\u3067\u304d\u308b\u3088\u3046\u306b\u3059\u308b\u3068\u3044\u3046\u76ee\u7684\u3067\u3001\u67fb\u8aad\u524d\u306e\u6bb5\u968e\u306e\u539f\u7a3f\u3092\u30aa\u30fc\u30d7\u30f3\u306b\u3059\u308b\u5236\u5ea6\u304c\u6574\u5099\u3055\u308c\u3066\u304d\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u7dd1\u8272\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea (green fluorescent protein, GFP)\u306f\u3001\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u86cd\u5149\u3067\u5149\u3089\u305b\u3066\u53ef\u8996\u5316\u3067\u304d\u308b\u3053\u3068\u304b\u3089\u3001\u751f\u547d\u79d1\u5b66\u7814\u7a76\u3067\u5e83\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305f\u3060\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3067\u304d\u3066\u3044\u308b\u3053\u3068\u304b\u3089\u3001\u52a0\u71b1\u306b\u3088\u308a\u5909\u6027\u3055\u305b\u308b\u3068\u86cd\u5149\u3092\u5931\u3046\u6027\u8cea\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u96fb\u6c17\u6cf3\u52d5\u6cd5\u3067\u3042\u308bSDS-PAGE\u3067\u5206\u96e2\u3057\u305f\u5f8c\u306b\u76ee\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093 (\u901a\u5e38\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u30dc\u30a4\u30eb\u3059\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f)\u3002\u4eca\u56de\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3067\u516c\u958b\u3057\u305f\u8ad6\u6587\u3067\u306f\u3001\u52a0\u71b1\u306b\u3088\u308a\u5909\u6027\u3057\u305fGFP\u3092SDS-PAGE\u5f8c\u306e\u30b2\u30eb\u5185\u3067\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3055\u305b\u308b\u7c21\u4fbf\u306a\u65b9\u6cd5\u3092\u5831\u544a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\n
\n
In-gel refolding allows fluorescence detection of fully denatured GFPs after SDS-PAGE<\/strong><\/div>\n<\/blockquote>\n<\/div>\n
\n
Misa<\/span> Shiratori<\/span><\/span>, Rio<\/span> Tsuyuki<\/span><\/span>, Miwako<\/span> Asanuma<\/span><\/span>, Saki<\/span> Kawabata<\/span><\/span>, Hiromasa<\/span> Yoshioka<\/span><\/span>, Kenji<\/span> Ohgane<\/span><\/span><\/span><\/div>\n
bioRxiv <\/span>2024.10.02.615947; <\/span>doi:<\/span> https:\/\/doi.org\/10.1101\/2024.10.02.615947<\/span><\/div>\n
https:\/\/www.biorxiv.org\/content\/10.1101\/2024.10.02.615947v1<\/a><\/div>\n<\/blockquote>\n
\"\"<\/div>\n
\u975e\u52a0\u71b1\u306eSDS-PAGE\u3084Native-PAGE\u306a\u3069\u306e\u96fb\u6c17\u6cf3\u52d5\u6cd5\u3067\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7acb\u4f53\u69cb\u9020\u3084\u4fee\u98fe\u72b6\u614b\u306a\u3069\u3055\u307e\u3056\u307e\u306a\u8981\u56e0\u3067\u79fb\u52d5\u5ea6\u304c\u5909\u5316\u3057\u307e\u3059\u304c\u3001\u901a\u5e38\u306e\u5909\u6027SDS-PAGE\u3067\u306f\u4e3b\u306b\u5206\u5b50\u91cf\u306b\u5fdc\u3058\u305f\u5206\u96e2\u304c\u3067\u304d\u308b\u3068\u3044\u3046\u5229\u70b9\u304c\u3042\u308a\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u3001GFP\u306e\u86cd\u5149\u691c\u51fa\u3092\u96fb\u6c17\u6cf3\u52d5\u5f8c\u306b\u884c\u3046\u5834\u5408\u3001\u975e\u52a0\u71b1SDS-PAGE\u3084Native-PAGE\u304c\u7528\u3044\u3089\u308c\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u300c\u5206\u5b50\u91cf\u300d\u901a\u308a\u306b\u6cf3\u52d5\u3055\u308c\u306a\u3044\u6b20\u70b9\u304c\u3042\u308a\u307e\u3059\u3002\u30b2\u30eb\u5185\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3092\u7528\u3044\u308b\u65b9\u6cd5\u3067\u306f\u3001\u3053\u306e\u6b20\u70b9\u3092\u56de\u907f\u3067\u304d\u307e\u3059\u3002<\/div>\n
\u73fe\u6642\u70b9\u3067\u306f\u3001EGFP\u3084GFPuv\u3001superfolder GFP (\u3068\u305d\u306emonomeric\u5909\u7570\u4f53)\u3001turboGFP\u306a\u3069\u3067\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u3067\u304d\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u3066\u304a\u308a\u3001turboGFP\u3068superfolder GFP (monomeric\u5909\u7570\u4f53)\u3067\u306f\u4ed6\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u306e\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u72b6\u614b\u3067\u3082\u30ea\u30d5\u30a9\u30fc\u30eb\u30c7\u30a3\u30f3\u30b0\u53ef\u80fd\u3067\u3059\u3002Total protein staining\u3084\u305d\u306e\u5f8c\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3068\u3082\u4f75\u7528\u53ef\u80fd\u306a\u306e\u3067\u3001\u7528\u9014\u30fb\u76ee\u7684\u306b\u3088\u3063\u3066\u306f\u4fbf\u5229\u306b\u4f7f\u3048\u308b\u65b9\u6cd5\u3067\u306f\u306a\u3044\u304b\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/div>\n
<\/div>\n
\u5927\u91d1<\/div>\n","protected":false},"excerpt":{"rendered":"

\u5927\u91d1\u7814\u306e\u7814\u7a76\u304c\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u30b5\u30fc\u30d0\u30fcbioRxiv\u3067\u516c\u958b\u3055\u308c\u307e\u3057\u305f\u3002B4\u767d\u9ce5\u3055\u3093\u3001M1\u9732\u6728\u3055\u3093\u3001\u5352\u696d\u751f\u306e\u5ddd\u7aef\u3055\u3093\u304c\u5bc4\u4e0e\u3057\u3066\u304f\u308c\u3066\u3044\u308b\u7814\u7a76\u3067\u3059\u3002 #\u306a\u304a\u3001\u30d7\u30ec\u30d7\u30ea\u30f3\u30c8\u3068\u306f\u3001\u5b66\u8853\u96d1\u8a8c\u3067\u306e\u67fb\u8aad\u524d\u306e\u539f\u7a3f\u3092\u516c\u958b\u3057\u305f\u3082\u306e\u3067\u3001\u5c02\u9580\u5bb6\u306b\u3088 … \u7d9a\u304d\u3092\u8aad\u3080 →<\/span><\/a><\/p>\n","protected":false},"author":48,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8],"tags":[],"class_list":["post-435","post","type-post","status-publish","format-standard","hentry","category-ohganelabnews"],"_links":{"self":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/435","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/users\/48"}],"replies":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/comments?post=435"}],"version-history":[{"count":1,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/435\/revisions"}],"predecessor-version":[{"id":437,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/435\/revisions\/437"}],"wp:attachment":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/media?parent=435"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/categories?post=435"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/tags?post=435"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}