{"id":167,"date":"2021-12-07T12:27:27","date_gmt":"2021-12-07T03:27:27","guid":{"rendered":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/?p=167"},"modified":"2021-12-08T09:39:05","modified_gmt":"2021-12-08T00:39:05","slug":"dna%e5%ae%9a%e9%87%8f%e3%81%ae%e3%83%97%e3%83%ad%e3%83%88%e3%82%b3%e3%83%ab%e3%82%92protocols-io%e3%81%a7%e5%85%ac%e9%96%8b%e3%81%97%e3%81%be%e3%81%97%e3%81%9f","status":"publish","type":"post","link":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2021\/12\/07\/dna%e5%ae%9a%e9%87%8f%e3%81%ae%e3%83%97%e3%83%ad%e3%83%88%e3%82%b3%e3%83%ab%e3%82%92protocols-io%e3%81%a7%e5%85%ac%e9%96%8b%e3%81%97%e3%81%be%e3%81%97%e3%81%9f\/","title":{"rendered":"DNA\u5b9a\u91cf\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3092protocols.io\u3067\u516c\u958b\u3057\u307e\u3057\u305f"},"content":{"rendered":"

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DNA\u306e\u5b9a\u91cf\u306b\u306f\u3001NanoDrop\u306a\u3069\u306e\u5fae\u91cf\u5206\u5149\u5149\u5ea6\u8a08\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u304c\u3001\u6570ng\/\u00b5L\u4ee5\u4e0b\u306eDNA\u306e\u5b9a\u91cf\u306f\u611f\u5ea6\u7684\u306b\u53b3\u3057\u304f\u306a\u3063\u3066\u304d\u307e\u3059\u3002\u3088\u308a\u9ad8\u611f\u5ea6\u306aDNA\u5b9a\u91cf\u6cd5\u3068\u3057\u3066\u3001\u4ee5\u524d\u304b\u3089DNA\u306b\u7d50\u5408\u3057\u3066\u86cd\u5149\u3092\u767a\u3059\u308b\u5316\u5408\u7269\u3092\u7528\u3044\u305f\u65b9\u6cd5\u304c\u77e5\u3089\u308c\u3066\u304a\u308a\u3001\u5909\u7570\u539f\u6027\u306e\u3042\u308bethidium bromide\u306a\u3069\u304c\u4f7f\u308f\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u3053\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f\u3001ethidium bromide\u3088\u308a\u5b89\u5168\u306a\u8a66\u85ac\u3068\u3057\u3066\u3001EzFluoroStain DNA (ATTO)\u3092\u7528\u3044\u3066DNA\u3092\u5b9a\u91cf\u3059\u308b\u65b9\u6cd5\u3092\u8aac\u660e\u3057\u3066\u3044\u307e\u3059\u3002\u985e\u4f3c\u306e\u65b9\u6cd5\u306fSYBR Green\u3084PicoGreen\u3068\u3044\u3063\u305f\u67d3\u8272\u8a66\u85ac\u3067\u3082\u5831\u544a\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u3053\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f\u5f53\u7814\u7a76\u5ba4\u3067\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u4e2d\u306eDNA\u67d3\u8272\u306b\u7528\u3044\u3066\u3044\u308bEzFluoroStain DNA\u3092\u4f7f\u3063\u3066\u5b9a\u91cf\u3059\u308b\u5b9f\u9a13\u6761\u4ef6\u3084\u5b9a\u91cf\u53ef\u80fd\u306a\u7bc4\u56f2\u306b\u3064\u3044\u3066\u3001\u7c21\u5358\u306b\u307e\u3068\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u306a\u304a\u3001\u30d7\u30ed\u30c8\u30b3\u30eb\u306e\u6761\u4ef6\u691c\u8a0e\u3084\u4f8b\u3068\u3057\u3066\u793a\u3057\u305f\u5b9a\u91cf\u30c7\u30fc\u30bf\u306e\u53d6\u5f97\u306f\u3001\u76f8\u5ddd\u7814\u7a76\u5ba4<\/a>\u304b\u3089\u5927\u91d1\u7814\u306b\u30d8\u30eb\u30d7\u3067\u6765\u3066\u304f\u308c\u3066\u3044\u308b\u9f4b\u7530\u8309\u5e06\u3055\u3093 (M1) \u304c\u884c\u306a\u3063\u3066\u304f\u308c\u307e\u3057\u305f\u3002\u3042\u308a\u304c\u3068\u3046\u3054\u3056\u3044\u307e\u3059\u3002<\/p>\n

Maho Saita, Kyoko Aikawa, Kenji Ohgane (2021) Microplate-based DNA Quantification with EzFluoroStain DNA reagent. protocols.io\u00a0<\/b>https:\/\/dx.doi.org\/10.17504\/protocols.io.bztvp6n6<\/a><\/p>\n

\"\"

protocols.io\u306b\u516c\u958b\u3057\u305fDNA\u5b9a\u91cf\u30d7\u30ed\u30c8\u30b3\u30eb<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

\u7814\u7a76\u5ba4\u3067\u30eb\u30fc\u30c1\u30f3\u306b\u884c\u3046\u5b9f\u9a13\u30d7\u30ed\u30c8\u30b3\u30eb\u3068\u3057\u3066\u3001\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u3067\u306e\u86cd\u5149\u6e2c\u5b9a\u306b\u3088\u308bDNA\u5b9a\u91cf\u6cd5\u306e\u30d7\u30ed\u30c8\u30b3\u30eb (\u30ea\u30f3\u30af\u5148\u306f\u82f1\u8a9e\u3067\u3059) \u3092protocols.io\u306b\u3066\u516c\u958b\u3057\u307e\u3057\u305f\u3002 DNA\u306e\u5b9a\u91cf\u306b\u306f\u3001NanoDrop\u306a\u3069\u306e\u5fae\u91cf\u5206 … \u7d9a\u304d\u3092\u8aad\u3080 →<\/span><\/a><\/p>\n","protected":false},"author":48,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[1],"tags":[],"class_list":["post-167","post","type-post","status-publish","format-standard","hentry","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/167","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/users\/48"}],"replies":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/comments?post=167"}],"version-history":[{"count":6,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/167\/revisions"}],"predecessor-version":[{"id":174,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/167\/revisions\/174"}],"wp:attachment":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/media?parent=167"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/categories?post=167"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/tags?post=167"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}