{"id":111,"date":"2021-10-02T12:59:36","date_gmt":"2021-10-02T03:59:36","guid":{"rendered":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/?p=111"},"modified":"2021-11-26T10:22:24","modified_gmt":"2021-11-26T01:22:24","slug":"b4%e5%b7%9d%e7%ab%af%e3%81%95%e3%82%93%e3%81%ae%e5%ae%9f%e9%a8%93%e7%b5%90%e6%9e%9c%e3%81%8coriciro-cell-free-cloning-system%e3%81%ae%e3%82%a2%e3%83%97%e3%83%aa%e3%82%b1%e3%83%bc%e3%82%b7%e3%83%a7","status":"publish","type":"post","link":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2021\/10\/02\/b4%e5%b7%9d%e7%ab%af%e3%81%95%e3%82%93%e3%81%ae%e5%ae%9f%e9%a8%93%e7%b5%90%e6%9e%9c%e3%81%8coriciro-cell-free-cloning-system%e3%81%ae%e3%82%a2%e3%83%97%e3%83%aa%e3%82%b1%e3%83%bc%e3%82%b7%e3%83%a7\/","title":{"rendered":"B4\u5ddd\u7aef\u3055\u3093\u306e\u5b9f\u9a13\u7d50\u679c\u304cOriCiro cell-free cloning system\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ce\u30fc\u30c8\u306b\u306a\u308a\u307e\u3057\u305f\u3002"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignright size-medium wp-image-113\" src=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/PA027740-300x225.jpg\" alt=\"\" width=\"300\" height=\"225\" srcset=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/PA027740-300x225.jpg 300w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/PA027740-1024x768.jpg 1024w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/PA027740-768x576.jpg 768w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/PA027740-1536x1152.jpg 1536w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/PA027740-2048x1536.jpg 2048w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>\u7d30\u80de\u306b\u8208\u5473\u5bfe\u8c61\u306e\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u306b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u305d\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f5c\u308b\u305f\u3081\u306b\u306f\u3001\u53e4\u5178\u7684\u306b\u306f\u5236\u9650\u9175\u7d20\u3084ligase\u3092\u7528\u3044\u3066DNA\u3092\u5207\u308a\u8cbc\u308a\u3057\u3001\u5927\u8178\u83cc\u3092\u7528\u3044\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5897\u3084\u3057\u3066\u7cbe\u88fd\u3059\u308b\u3001\u3068\u3044\u3046\u6d41\u308c\u306e\u5b9f\u9a13\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n<p>\u3054\u304f\u7a00\u306b\u3001\u5927\u8178\u83cc\u3067\u5897\u3084\u3059\u3053\u3068\u304c\u96e3\u3057\u3044\u907a\u4f1d\u5b50\u304c\u3042\u308a\u307e\u3059\u3002\u5927\u91d1\u7814\u3067\u7814\u7a76\u5bfe\u8c61\u3068\u3057\u3066\u3044\u308b\u3001\u907a\u4f1d\u6027\u96e3\u75c5\u30cb\u30fc\u30de\u30f3\u30d4\u30c3\u30af\u75c5C\u578b\u306e\u539f\u56e0\u3068\u306a\u308bNPC1\u3068\u3044\u3046\u907a\u4f1d\u5b50\u3082\u305d\u306e\u4e00\u4f8b\u3067\u3059\u3002\u3053\u308c\u307e\u3067\u3001\u3053\u306e\u907a\u4f1d\u5b50\u3092\u542b\u3080\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u5927\u8178\u83cc\u5185\u3067\u4e0d\u5b89\u5b9a\u3067\u3001\u7d44\u63db\u3048\u306f\u304b\u306a\u308a\u5927\u5909\u306a\u4f5c\u696d\u3067\u3057\u305f\u3002<\/p>\n<p>2020\u5e74\u306b\u306a\u308a\u3001\u7acb\u6559\u5927\u5b66\u306e\u672b\u6b21\u7814\u7a76\u5ba4\u3067\u958b\u767a\u3055\u308c\u305f\u300c\u5927\u8178\u83cc\u3092\u4f7f\u308f\u306a\u3044\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u6cd5\u300d\u304c\u30aa\u30ea\u30b7\u30ed\u30b8\u30a7\u30ce\u30df\u30af\u30b9\u793e\u304b\u3089\u30ad\u30c3\u30c8\u5316\u3055\u308c\u3066\u8ca9\u58f2\u304c\u958b\u59cb\u3055\u308c\u307e\u3057\u305f\u3002\u3053\u306e\u65b9\u6cd5\u306f\u3001\u5927\u8178\u83cc\u5185\u3067\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u8907\u88fd\u3055\u308c\u3066\u3044\u304f\u4ed5\u7d44\u307f\u3092\u8a66\u9a13\u7ba1\u5185\u3067\u518d\u69cb\u6210\u3057\u305f\u5b9f\u9a13\u7cfb\u3067\u3001\u65e5\u672c\u767a\u306e\u65b0\u3057\u3044\u6280\u8853\u3067\u3059\u3002\u4e0a\u8a18\u306eNPC1\u306f\u300c\u5927\u8178\u83cc\u5185\u3067\u300d\u5897\u3084\u3059\u306e\u304c\u96e3\u3057\u3044\u907a\u4f1d\u5b50\u3060\u3063\u305f\u8a33\u3067\u3059\u304b\u3089\u3001\u3053\u306e\u518d\u69cb\u6210\u7cfb\u3067\u3042\u308c\u3070\u554f\u984c\u306a\u304f\u5897\u3084\u3057\u305f\u308a\u7d44\u63db\u3048\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u305d\u306e\u3088\u3046\u306a\u80cc\u666f\u3067\u3001NPC1\u3092\u542b\u3080\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u7d44\u63db\u3048\u306b\u3053\u306eOriCiro cell-free cloning system\u3092\u4f7f\u3063\u3066\u8a66\u3057\u3066\u307f\u307e\u3057\u305f\u3002B4\u306e\u5ddd\u7aef\u3055\u3093\u304c\u8a66\u3057\u3066\u304f\u308c\u307e\u3057\u3066\u3001\u76ee\u7684\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u4f5c\u6210\u306f\u3046\u307e\u304f\u3067\u304d\u3001\u305d\u306e\u7d50\u679c\u3092\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ce\u30fc\u30c8\u3068\u3057\u3066\u60c5\u5831\u5171\u6709\u3092\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30ce\u30fc\u30c8\u300cNPC1-\u30eb\u30b7\u30d5\u30a7\u30e9\u30fc\u30bc\u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u7bc9\u3068\u5897\u5e45\u300d\u306f\u3001\u4e0b\u8a18\u304b\u3089\u8aad\u3081\u307e\u3059\u306e\u3067\u3001\u8208\u5473\u304c\u3042\u3063\u305f\u3089\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n<ul>\n<li><a href=\"https:\/\/www.oriciro.com\/appnote\">OriCiro Genomics\u306eApplication Notes<\/a>\u00a0(in English)<\/li>\n<li>\u30d5\u30ca\u30b3\u30b7\u306eOriCiro cell-free cloning system\u306e\u30da\u30fc\u30b8 (\u300c\u30e6\u30fc\u30b6\u30fc\u306e\u58f0\u300d\u306e\u3068\u3053\u308d) <a href=\"https:\/\/www.funakoshi.co.jp\/contents\/68153\">https:\/\/www.funakoshi.co.jp\/contents\/68153 (\u65e5\u672c\u8a9e)<\/a><\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.oriciro.com\/appnote\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-139\" src=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes.png\" alt=\"\" width=\"600\" height=\"311\" srcset=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes.png 1118w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes-300x156.png 300w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes-1024x531.png 1024w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes-768x398.png 768w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes-24x12.png 24w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes-36x19.png 36w, https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-content\/uploads\/sites\/31\/2021\/10\/OriCiro_ApplicationNotes-48x25.png 48w\" sizes=\"auto, (max-width: 600px) 100vw, 600px\" \/><\/a><\/p>\n<p style=\"text-align: right\">\u5927\u91d1<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u7d30\u80de\u306b\u8208\u5473\u5bfe\u8c61\u306e\u907a\u4f1d\u5b50\u3092\u767a\u73fe\u3055\u305b\u308b\u305f\u3081\u306b\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u305d\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4f5c\u308b\u305f\u3081\u306b\u306f\u3001\u53e4\u5178\u7684\u306b\u306f\u5236\u9650\u9175\u7d20\u3084ligase\u3092\u7528\u3044\u3066DNA\u3092\u5207\u308a\u8cbc\u308a\u3057\u3001\u5927\u8178\u83cc\u3092\u7528\u3044\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u5897\u3084\u3057\u3066\u7cbe\u88fd\u3059\u308b\u3001\u3068\u3044\u3046\u6d41\u308c\u306e\u5b9f\u9a13\u3092\u884c\u3044 &hellip; <a href=\"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/2021\/10\/02\/b4%e5%b7%9d%e7%ab%af%e3%81%95%e3%82%93%e3%81%ae%e5%ae%9f%e9%a8%93%e7%b5%90%e6%9e%9c%e3%81%8coriciro-cell-free-cloning-system%e3%81%ae%e3%82%a2%e3%83%97%e3%83%aa%e3%82%b1%e3%83%bc%e3%82%b7%e3%83%a7\/\">\u7d9a\u304d\u3092\u8aad\u3080 <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":48,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[8,1],"tags":[],"class_list":["post-111","post","type-post","status-publish","format-standard","hentry","category-ohganelabnews","category-uncategorized"],"_links":{"self":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/111","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/users\/48"}],"replies":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/comments?post=111"}],"version-history":[{"count":7,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/111\/revisions"}],"predecessor-version":[{"id":165,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/posts\/111\/revisions\/165"}],"wp:attachment":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/media?parent=111"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/categories?post=111"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/ohganelab\/wp-json\/wp\/v2\/tags?post=111"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}