{"id":138,"date":"2015-08-03T13:30:43","date_gmt":"2015-08-03T04:30:43","guid":{"rendered":"http:\/\/www-p.cc.ocha.ac.jp\/bio-shimada-lab\/?page_id=138"},"modified":"2015-08-03T13:30:43","modified_gmt":"2015-08-03T04:30:43","slug":"pr_04freeze","status":"publish","type":"page","link":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/ex_protocol\/pr_04freeze.html","title":{"rendered":"\u51cd\u7d50\u4fdd\u5b58\uff08\u4e8c\u6bb5\u968e\u51cd\u7d50\uff09\u6cd5"},"content":{"rendered":"
    \n
  1. \u30d7\u30ed\u30b0\u30e9\u30e0\u30d5\u30ea\u30fc\u30b6\u30fc\u3092\u7528\u3044\u3066\u5f90\u3005\u306b-40\u2103\u307e\u3067\u4e0b\u3052\u305f\u5f8c\u3001\u6db2\u4f53\u7a92\u7d20\u3067-196\u2103\u307e\u3067\u6025\u901f\u51cd\u7d50\u3055\u305b\u308b\u3001\u4e8c\u6bb5\u968e\u51cd\u7d50\u6cd5\u3002
    \nDMSO\u3068\u57f9\u5730\u3092\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u6df7\u5408\u3057\u3001\u500d\u6fc3\u5ea6\u306eDMSO\u6eb6\u6db2\u3092\u8abf\u6574\u3002\u6c37\u3067\u51b7\u3084\u3057\u3066\u304a\u304f\uff08\u6df7\u5408\u6642\u306b\u767a\u71b1\u3059\u308b\u305f\u3081\uff09\u3002
    \n\u4f8b\uff1a\u30003\uff05\u3067\u51cd\u7d50\u3059\u308b\u5834\u5408\u3001\uff16\uff05DMSO\u6eb6\u6db2\u3092\u4f5c\u6210
    \n\u30c1\u30e5\u30fc\u30d6 30\u672c\u5206\u3000DMSO\uff1a960\u03bcl \u57f9\u5730\uff1a15,040\u03bcl
    \n20\u672c\u5206 720 11,280
    \n10\u672c\u5206\u3000\u3000\u3000\u3000\u3000 360 5,640<\/li>\n
  2. \uff30\u30d5\u30ea\u30fc\u30b6\u30fc\u306e\u7a92\u7d20\u30bf\u30f3\u30af\u306b\u30dd\u30f3\u30d7\u3092\u30bb\u30c3\u30c8\u3057\u3001\u6c17\u5727\u8abf\u7bc0\u5f01\u3092\u9589\u3081\u3066\u5727\u3092\u4e0a\u3052\u3066\u304a\u304f\u3002<\/li>\n
  3. \u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u306b\u30b5\u30f3\u30d7\u30eb\u3092500\u03bcl\u305a\u3064\u5206\u6ce8\u3002<\/li>\n
  4. \u8abf\u6574\u6e08DMSO\u6eb6\u6db2\u3092500\u03bcl\u305a\u3064\u6ce8\u5165\u3057\u6df7\u5408\u3001\u5ba4\u6e29\u306715\u5206\u9593\u304a\u304f\u3002<\/li>\n
  5. \u305d\u306e\u9593\u306b\u3001P\u30d5\u30ea\u30fc\u30b6\u30fc\u3092\u30bb\u30c3\u30c6\u30a3\u30f3\u30b0\u3002\u5404\u30c1\u30e5\u30fc\u30d6\u306b\u30d0\u30fc\u30b3\u30fc\u30c9\u30b7\u30fc\u30eb\u3092\u5f35\u308b\uff08\u307e\u305f\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u540d\u3068\u65e5\u4ed8\u3092\u76f4\u63a5\u8a18\u5165\uff09\u3002<\/li>\n
  6. P\u30d5\u30ea\u30fc\u30b6\u30fc\u306e\u6e96\u5099\u304c\u3067\u304d\u305f\u3089\u3001\u30b1\u30fc\u30f3\u306b\u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u30bb\u30c3\u30c8\u3057\u3066\u5165\u308c\u3001\u30b9\u30bf\u30fc\u30c8\u3055\u305b\u308b\u3002<\/li>\n
  7. \u6e29\u5ea6\u8a18\u9332\u8a08\u306e\u30b9\u30a4\u30c3\u30c1\u3092\u5165\u308c\u308b\u3002<\/li>\n
  8. \u300c\uff0d40\u2103\u307e\u3067\u6bce\u52061\u2103\u3065\u3064\u51b7\u5374\u3057\u3001\uff0d40\u2103\u306715\u5206\u4fdd\u6301\u300d\u306e\u30d7\u30ed\u30b0\u30e9\u30e0\u3067\u904b\u8ee2\u3002<\/li>\n
  9. 75\u5206\u3067\u7d42\u4e86\u3059\u308b\u306e\u3067\u3001\u305d\u306e\u524d\u306b\u30c7\u30e5\u30a2\u30fc\u74f6\u306b\u6db2\u4f53\u7a92\u7d20\u3092\u5165\u308c\u3066\u304a\u304f\u3002<\/li>\n
  10. \u30d7\u30ed\u30b0\u30e9\u30e0\u304c\u7d42\u4e86\u3057\u305f\u3089\u3001\u7d20\u65e9\u304f\u30b1\u30fc\u30f3\u304b\u3089\u30c1\u30e5\u30fc\u30d6\u3092\u5916\u3057\u3066\u6db2\u4f53\u7a92\u7d20\u306b\u3064\u3051\u308b\uff08\u6025\u901f\u51cd\u7d50\u6cd5\uff09\u3002<\/li>\n
  11. P\u30d5\u30ea\u30fc\u30b6\u30fc\u306f\u30c1\u30e3\u30f3\u30d0\u30fc\u309220\u2103\u306b\u623b\u3057\u3066\u304b\u3089\u96fb\u6e90\u3092\u5207\u308b\u3002\u7a92\u7d20\u30dd\u30f3\u30d7\u306e\u5f01\u3092\u958b\u3051\u3001\u6c17\u5727\u304c0\u307e\u3067\u4e0b\u304c\u3063\u305f\u306e\u3092\u78ba\u8a8d\u3057\u305f\u3089\u30dd\u30f3\u30d7\u3092\u53d6\u308a\u5916\u3059\u3002<\/li>\n
  12. \u30af\u30e9\u30a4\u30aa\u30c1\u30e5\u30fc\u30d6\u3092\u6db2\u4f53\u7a92\u7d20\u4fdd\u5b58\u69fd\uff08\u6c17\u76f8\u4fdd\u5b58\u3001\u7d04\uff0d160\u2103\uff09\u5185\u306b\u53ce\u7d0d\u3002<\/li>\n
  13. \u89e3\u51cd\u6642\u3001\u30a6\u30a9\u30fc\u30bf\u30fc\u30d0\u30b9\u309239.5\u2103\u306b\u8a2d\u5b9a\u3057\u6696\u3081\u3066\u304a\u304f\uff0837?40\u2103\u304c\u826f\u3044\uff09\u3002<\/li>\n
  14. \u30b1\u30fc\u30f3\u3084\u30d4\u30f3\u30bb\u30c3\u30c8\u3092\u7528\u3044\u3001\u3059\u3070\u3084\u304f\u632f\u308a\u306a\u304c\u3089\u6700\u5f8c\u306e\u6c37\u304c\u89e3\u3051\u308b\u307e\u3067\u89e3\u51cd\u3059\u308b\u3002<\/li>\n
  15. \u57f9\u5730\u30672\u56de\u307b\u3069\u6d17\u6d44\u3057\u3066\uff08\u30b5\u30f3\u30d7\u30eb\u304c\u5fae\u5c0f\u306a\u5834\u5408\u306f\u9060\u5fc3\u6a5f\u3092\u4f7f\u3046\uff09\u3001\u57f9\u990a\u30c1\u30e5\u30fc\u30d6\u306b\u690d\u3048\u8fbc\u3080\u3002<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u30d7\u30ed\u30b0\u30e9\u30e0\u30d5\u30ea\u30fc\u30b6\u30fc\u3092\u7528\u3044\u3066\u5f90\u3005\u306b-40\u2103\u307e\u3067\u4e0b\u3052\u305f\u5f8c\u3001\u6db2\u4f53\u7a92\u7d20\u3067-196\u2103\u307e\u3067\u6025\u901f\u51cd\u7d50\u3055\u305b\u308b\u3001\u4e8c\u6bb5\u968e\u51cd\u7d50\u6cd5\u3002 DMSO\u3068\u57f9\u5730\u3092\u9060\u5fc3\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u6df7\u5408\u3057\u3001\u500d\u6fc3\u5ea6\u306eDMSO\u6eb6\u6db2\u3092\u8abf\u6574\u3002\u6c37\u3067\u51b7\u3084\u3057\u3066\u304a\u304f\uff08\u6df7\u5408\u6642\u306b\u767a\u71b1\u3059\u308b\u305f\u3081\uff09 … \u7d9a\u304d\u3092\u8aad\u3080 →<\/span><\/a><\/p>\n","protected":false},"author":18,"featured_media":0,"parent":94,"menu_order":4,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-138","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/pages\/138","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/users\/18"}],"replies":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/comments?post=138"}],"version-history":[{"count":0,"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/pages\/138\/revisions"}],"up":[{"embeddable":true,"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/pages\/94"}],"wp:attachment":[{"href":"https:\/\/www-p.sci.ocha.ac.jp\/bio-shimada-lab\/wp-json\/wp\/v2\/media?parent=138"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}