{"id":528,"date":"2015-08-25T15:43:13","date_gmt":"2015-08-25T06:43:13","guid":{"rendered":"http:\/\/www-p.cc.ocha.ac.jp\/chem-ogawa\/?page_id=528"},"modified":"2015-08-25T15:43:13","modified_gmt":"2015-08-25T06:43:13","slug":"kenyu_5","status":"publish","type":"page","link":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/theme\/kenyu_5.html","title":{"rendered":"5. \u5171\u751f\u30fb\u5bc4\u751f\u306b\u95a2\u308f\u308b\u7cd6\u9396"},"content":{"rendered":"

\u25cf\u30ad\u30ce\u30b3\u30ec\u30af\u30c1\u30f3\u306e\u591a\u91cd\u7d50\u5408\u7279\u7570\u6027\u3000multispecificity<\/p>\n

\u83cc\u985e\u306e\u4e2d\u3067\u3082\u3001\u30ad\u30ce\u30b3\u306f\u4e0d\u601d\u8b70\u306a\u5b58\u5728\u3067\u3059\u3002\u4f55\u3082\u306a\u3044\u8150\u3063\u305f\u571f\u3084\u67af\u308c\u8449\u306e\u4e2d\u304b\u3089\u3072\u3087\u3063\u3053\u308a\u3068\u751f\u3048\u3066\u304f\u308b\u3088\u3046\u306b\u898b\u3048\u307e\u3059\u3002\u306a\u305c\u304b\u30ad\u30ce\u30b3\u306b\u306f\u69d8\u3005\u306a\u30e6\u30cb\u30fc\u30af\u306a\u7279\u7570\u6027\u3092\u6301\u3064\u7cd6\u7d50\u5408\u30bf\u30f3\u30d1\u30af\u8cea\uff08\u30ec\u30af\u30c1\u30f3\uff09\u304c\u5b58\u5728\u3057\u3066\u3044\u307e\u3059 3)<\/sup> \u3002\u771f\u83cc\u985e\u306e\u4e00\u7a2e\u3001\u62c5\u5b50\u83cc\u985e\u306e\u30e0\u30b8\u30ca\u30bf\u30b1\u30ec\u30af\u30c1\u30f3(PVL)\u306f\u3001\u7cd6\u9396\u306e\u975e\u9084\u5143\u672b\u7aef\uff2e\uff0d\u30a2\u30bb\u30c1\u30eb\u30b0\u30eb\u30b3\u30b5\u30df\u30f3 (GlcNAc)\u306b\u7279\u7570\u7684\u306a\u6570\u5c11\u306a\u3044\u30ec\u30af\u30c1\u30f3\u306e\u4e00\u3064\u3067\u3001\u751f\u5316\u5b66\u3084\u75c5\u7406\u5b66\u65b9\u9762\u3067\u306e\u7cd6\u9396\u7814\u7a76\u306b\u3001\u307e\u305f\u81e8\u5e8a\u8a3a\u65ad\u85ac\u3078\u306e\u6709\u7528\u6027\u304c\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u79c1\u9054\u306fPVL\u304c GlcNAc\u7d50\u5408\u90e8\u4f4d\u3068\u540c\u3058\u90e8\u4f4d\u3067\uff2e\uff0d\u30a2\u30bb\u30c1\u30eb\u57fa\u3092\u6301\u3064\u4ed6\u306e\u7cd6\u3001\u30b7\u30a2\u30eb\u9178 4)<\/sup> \u306a\u3069\u306b\u3082\u826f\u304f\u7d50\u5408\u3059\u308b\u3053\u3068 1)<\/sup> \u3001\u307e\u305f\u30d8\u30d1\u30ea\u30f3\u3084\u30dd\u30ea\u30ac\u30e9\u30af\u30c4\u30ed\u30f3\u9178\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u9178\u6027\u591a\u7cd6\u3068\u3082\u7d50\u5408\u3059\u308b\u304c\u3001\u3053\u308c\u306fGlcNAc\u8a8d\u8b58\u90e8\u4f4d\u3068\u7570\u306a\u308b\u90e8\u4f4d\u3067\u306e\u76f8\u4e92\u4f5c\u7528\u3067\u3042\u308b\u3053\u3068\u3092\u898b\u3044\u51fa\u3057\u307e\u3057\u305f 2)<\/sup> \u3002\u3059\u306a\u308f\u3061PVL\u306fmultispecific\u306a\u30ec\u30af\u30c1\u30f3\u3067\u3001\u3053\u306e\u4ed6\u306b\u306b\u3082\u9178\u6027\u7cd6\u8102\u8cea\u306a\u3069\u69d8\u3005\u306a\u7cd6\u9396\u3092\u6301\u3064\u751f\u4f53\u9ad8\u5206\u5b50\u3068\u7d50\u5408\u3067\u304d\u308b\u6027\u8cea\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002PVL\u304c\u8150\u751f\u83cc\u3067\u3042\u308b\u30e0\u30b8\u30ca\u30bf\u30b1\u751f\u4f53\u5185\u3067\u3069\u3093\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u306e\u304b\u3001\u307e\u3060\u3088\u304f\u308f\u304b\u308a\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u4ed6\u306e\u771f\u83cc\u985e\u3067\u3042\u308b Arthrobotrys oligospora \u3067\u306f\u3001\u83cc\u304c\u7dda\u866b\u3092\u6355\u98df\u3059\u308b\u3068\u304d\u306bmultispecific\u306a\u30ec\u30af\u30c1\u30f3\u304c\u50cd\u3044\u3066\u3044\u308b\u53ef\u80fd\u6027\u3082\u8003\u3048\u3089\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u3053\u306e\u3088\u3046\u306a\u30ec\u30af\u30c1\u30f3\u304c\u81ea\u7136\u754c\u3067\u5bc4\u751f\u3001\u8150\u751f\u3001\u5171\u751f\u3092\u4e2d\u5fc3\u3068\u3057\u305f\u83cc\u985e\u306e\u751f\u614b\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3057\u3066\u3044\u308b\u306e\u3067\u306f\u306a\u3044\u304b\u3068\u671f\u5f85\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

    \n
  1. Ueda H., et al., FEBS Lett.<\/i> , 448(1),75-80, (1999)
    \nInteraction of a lectin from Psathyrella velutina mushroom with N-acetylneuraminic acid<\/a><\/li>\n
  2. Ueda H. et al., J. Biochem.<\/i> , 126(3), 530-537, (1999)
    \n    Multi-specificity of a Psathyrella velutina mushroom lectin: heparin\/pectin binding occurs at a site different from the N-acetylglucosamine\/N-acetylneuraminic acid-specific site<\/a><\/li>\n
  3. Matsumoto H., et al., Biochem. Biophys. Acta<\/i> , 1526(1), 37-43, (2001)
    \n    Screening of a unique lectin from 16 cultivable mushrooms with hybrid glycoprotein and neoproteoglycan probes and purification of a novel N-acetylglucosamine-specific lectin from Oudemansiella platyphylla fruiting body<\/a><\/li>\n
  4. Ueda H., et al., J. Biol. Chem.<\/i> , 277(28),24916-24925, (2002)
    \n    Psathyrella velutina Mushroom Lectin Exhibits High Affinity toward Sialoglycoproteins Possessing Terminal N-Acetylneuraminic Acid alpha 2,3-Linked to Penultimate Galactose Residues of Trisialyl N-Glycans. Comparison with other sialic acid-specific lectins<\/a><\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    \u25cf\u30ad\u30ce\u30b3\u30ec\u30af\u30c1\u30f3\u306e\u591a\u91cd\u7d50\u5408\u7279\u7570\u6027\u3000multispecificity \u83cc\u985e\u306e\u4e2d\u3067\u3082\u3001\u30ad\u30ce\u30b3\u306f\u4e0d\u601d\u8b70\u306a\u5b58\u5728\u3067\u3059\u3002\u4f55\u3082\u306a\u3044\u8150\u3063\u305f\u571f\u3084\u67af\u308c\u8449\u306e\u4e2d\u304b\u3089\u3072\u3087\u3063\u3053\u308a\u3068\u751f\u3048\u3066\u304f\u308b\u3088\u3046\u306b\u898b\u3048\u307e\u3059\u3002\u306a\u305c\u304b\u30ad\u30ce\u30b3\u306b\u306f\u69d8\u3005\u306a\u30e6\u30cb\u30fc\u30af\u306a\u7279\u7570\u6027\u3092\u6301 … \u7d9a\u304d\u3092\u8aad\u3080 →<\/span><\/a><\/p>\n","protected":false},"author":17,"featured_media":0,"parent":14,"menu_order":5,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-528","page","type-page","status-publish","hentry"],"aioseo_notices":[],"_links":{"self":[{"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/pages\/528","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/users\/17"}],"replies":[{"embeddable":true,"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/comments?post=528"}],"version-history":[{"count":0,"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/pages\/528\/revisions"}],"up":[{"embeddable":true,"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/pages\/14"}],"wp:attachment":[{"href":"http:\/\/www-p.sci.ocha.ac.jp\/chem-ogawa\/wp-json\/wp\/v2\/media?parent=528"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}